A protein interaction map of Drosophila melanogaster.

@article{citeulike:227121, abstract = {Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.}, address = {CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA.}, author = {Giot, L. and Bader, J. S. and Brouwer, C. and Chaudhuri, A. and Kuang, B. and Li, Y. and Hao, Y. L. and Ooi, C. E. and Godwin, B. and Vitols, E. and Vijayadamodar, G. and Pochart, P. and Machineni, H. and Welsh, M. and Kong, Y. and Zerhusen, B. and Malcolm, R. and Varrone, Z. and Collis, A. and Minto, M. and Burgess, S. and McDaniel, L. and Stimpson, E. and Spriggs, F. and Williams, J. and Neurath, K. and Ioime, N. and Agee, M. and Voss, E. and Furtak, K. and Renzulli, R. and Aanensen, N. and Carrolla, S. and Bickelhaupt, E. and Lazovatsky, Y. and DaSilva, A. and Zhong, J. and Stanyon, C. A. and Finley, R. L. and White, K. P. and Braverman, M. and Jarvie, T. and Gold, S. and Leach, M. and Knight, J. and Shimkets, R. A. and McKenna, M. P. and Chant, J. and Rothberg, J. M. }, citeulike-article-id = {227121}, doi = {10.1126/science.1090289}, issn = {1095-9203}, journal = {Science}, keywords = {drosophila, fly, high-throughput, interaction, map, protein-protein}, month = {December}, number = {5651}, pages = {1727--1736}, posted-at = {2006-11-01 23:37:21}, priority = {3}, title = {A protein interaction map of Drosophila melanogaster.}, url = {http://dx.doi.org/10.1126/science.1090289}, volume = {302}, year = {2003} }

TY - JOUR ID - citeulike:227121 L3 - citeulike-article-id:227121 TI - A protein interaction map of Drosophila melanogaster. JF - Science VL - 302 IS - 5651 SP - 1727 EP - 1736 AD - CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA. SN - 1095-9203 N2 - Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans. KW - drosophila KW - fly KW - high-throughput KW - interaction KW - map KW - protein-protein AU - Giot, L AU - Bader, JS AU - Brouwer, C AU - Chaudhuri, A AU - Kuang, B AU - Li, Y AU - Hao, YL AU - Ooi, CE AU - Godwin, B AU - Vitols, E AU - Vijayadamodar, G AU - Pochart, P AU - Machineni, H AU - Welsh, M AU - Kong, Y AU - Zerhusen, B AU - Malcolm, R AU - Varrone, Z AU - Collis, A AU - Minto, M AU - Burgess, S AU - McDaniel, L AU - Stimpson, E AU - Spriggs, F AU - Williams, J AU - Neurath, K AU - Ioime, N AU - Agee, M AU - Voss, E AU - Furtak, K AU - Renzulli, R AU - Aanensen, N AU - Carrolla, S AU - Bickelhaupt, E AU - Lazovatsky, Y AU - DaSilva, A AU - Zhong, J AU - Stanyon, CA AU - Finley, RL AU - White, KP AU - Braverman, M AU - Jarvie, T AU - Gold, S AU - Leach, M AU - Knight, J AU - Shimkets, RA AU - McKenna, MP AU - Chant, J AU - Rothberg, JM PY - 2003/12/05/ UR - http://dx.doi.org/10.1126/science.1090289 ER -