Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism.

by: K Kellner, C Heude-Berthelin, M Mathieu

Tissue & cell, Vol. 34, No. 2. (April 2002), pp. 109-116.

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Abstract

Immunological detection of glucagon-like peptides was performed in the cerebral ganglia of the mussel Mytilus edulis using an anti-vertebrate glucagon antibody. Two clusters of positive neurosecretory cells were observed, as well as stained nervous fibers. The effect of vertebrate glucagon on glucose incorporation into glycogen of reserve cells was tested using an in vitro microplate bioassay. Optimal incubation conditions were previously defined and an inhibitory effect of porcine glucagon was obtained for concentrations ranging from 10(-6) to 10(-9)M. It is postulated that the glucagon-like peptide may be implicated in the regulation of glucose metabolism in bivalves.

@article{citeulike:2861461, abstract = {Immunological detection of glucagon-like peptides was performed in the cerebral ganglia of the mussel Mytilus edulis using an anti-vertebrate glucagon antibody. Two clusters of positive neurosecretory cells were observed, as well as stained nervous fibers. The effect of vertebrate glucagon on glucose incorporation into glycogen of reserve cells was tested using an in vitro microplate bioassay. Optimal incubation conditions were previously defined and an inhibitory effect of porcine glucagon was obtained for concentrations ranging from 10(-6) to 10(-9)M. It is postulated that the glucagon-like peptide may be implicated in the regulation of glucose metabolism in bivalves.}, address = {Laboratoire de Biologie et Biotechnologies Marines, Universit\'{e} de Caen, Esplanade de la paix, 14032 Caen Cedex, France. kellner@ibba.unicaen.fr}, author = {Kellner, K. and Heude-Berthelin, C. and Mathieu, M. }, citeulike-article-id = {2861461}, issn = {0040-8166}, journal = {Tissue \& cell}, keywords = {glycogen-metabolism, mytilus, neuron}, month = {April}, number = {2}, pages = {109--116}, posted-at = {2008-06-04 16:22:29}, priority = {0}, title = {Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12165246}, volume = {34}, year = {2002} }

RIS record

TY - JOUR ID - citeulike:2861461 L3 - citeulike-article-id:2861461 N2 - Immunological detection of glucagon-like peptides was performed in the cerebral ganglia of the mussel Mytilus edulis using an anti-vertebrate glucagon antibody. Two clusters of positive neurosecretory cells were observed, as well as stained nervous fibers. The effect of vertebrate glucagon on glucose incorporation into glycogen of reserve cells was tested using an in vitro microplate bioassay. Optimal incubation conditions were previously defined and an inhibitory effect of porcine glucagon was obtained for concentrations ranging from 10(-6) to 10(-9)M. It is postulated that the glucagon-like peptide may be implicated in the regulation of glucose metabolism in bivalves. IS - 2 JF - Tissue & cell SN - 0040-8166 AD - Laboratoire de Biologie et Biotechnologies Marines, Université de Caen, Esplanade de la paix, 14032 Caen Cedex, France. kellner@ibba.unicaen.fr EP - 116 TI - Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism. VL - 34 SP - 109 KW - glycogen-metabolism KW - mytilus KW - neuron AU - Kellner, K AU - Heude-Berthelin, C AU - Mathieu, M PY - 2002/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/12165246 ER -

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