High-frequency gene transfer from the chloroplast genome to the nucleus

@article{citeulike:1203272, abstract = {Eukaryotic cells arose through endosymbiotic uptake of free-living bacteria followed by massive gene transfer from the genome of the endosymbiont to the host nuclear genome. Because this gene transfer took place over a time scale of hundreds of millions of years, direct observation and analysis of primary transfer events has remained difficult. Hence, very little is known about the evolutionary frequency of gene transfer events, the size of transferred genome fragments, the molecular mechanisms of the transfer process, or the environmental conditions favoring its occurrence. We describe here a genetic system based on transgenic chloroplasts carrying a nuclear selectable marker gene that allows the efficient selection of plants with a nuclear genome that carries pieces transferred from the chloroplast genome. We can select such gene transfer events from a surprisingly small population of plant cells, indicating that the escape of genetic material from the chloroplast to the nuclear genome occurs much more frequently than generally believed and thus may contribute significantly to intraspecific and intraorganismic genetic variation. 10.1073/pnas.1430924100}, author = {Stegemann, Sandra and Hartmann, Stefanie and Ruf, Stephanie and Bock, Ralph }, citeulike-article-id = {1203272}, comment = {Bot\^{a}nica Marinha Aula: Endossimbioses}, doi = {http://dx.doi.org/10.1073/pnas.1430924100}, journal = {PNAS}, keywords = {endosymbiosis, evolution, plastids}, month = {July}, number = {15}, pages = {8828--8833}, posted-at = {2007-04-02 19:26:36}, priority = {0}, title = {High-frequency gene transfer from the chloroplast genome to the nucleus}, url = {http://dx.doi.org/10.1073/pnas.1430924100}, volume = {100}, year = {2003} }

TY - JOUR ID - citeulike:1203272 L3 - citeulike-article-id:1203272 N2 - Eukaryotic cells arose through endosymbiotic uptake of free-living bacteria followed by massive gene transfer from the genome of the endosymbiont to the host nuclear genome. Because this gene transfer took place over a time scale of hundreds of millions of years, direct observation and analysis of primary transfer events has remained difficult. Hence, very little is known about the evolutionary frequency of gene transfer events, the size of transferred genome fragments, the molecular mechanisms of the transfer process, or the environmental conditions favoring its occurrence. We describe here a genetic system based on transgenic chloroplasts carrying a nuclear selectable marker gene that allows the efficient selection of plants with a nuclear genome that carries pieces transferred from the chloroplast genome. We can select such gene transfer events from a surprisingly small population of plant cells, indicating that the escape of genetic material from the chloroplast to the nuclear genome occurs much more frequently than generally believed and thus may contribute significantly to intraspecific and intraorganismic genetic variation. 10.1073/pnas.1430924100 IS - 15 JF - PNAS EP - 8833 TI - High-frequency gene transfer from the chloroplast genome to the nucleus VL - 100 SP - 8828 KW - endosymbiosis KW - evolution KW - plastids N1 - Botânica Marinha Aula: Endossimbioses AU - Stegemann, Sandra AU - Hartmann, Stefanie AU - Ruf, Stephanie AU - Bock, Ralph PY - 2003/07/22/ UR - http://dx.doi.org/10.1073/pnas.1430924100 ER -