DNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco

@article{citeulike:1203260, abstract = {By transforming chloroplasts with an antibiotic-resistance gene under the control of a nuclear-specific promoter, we employed a selection scheme to detect the transfer of DNA from the chloroplast to the nucleus in the green alga Chlamydomonas reinhardtii. Among several billion homoplasmic cells tested, we were unable to detect any stable nuclear integration of chloroplast DNA under normal growth conditions or under stress conditions. This contrasts with results reported for the transfer of DNA from chloroplast to nucleus in higher plants and from mitochondrion to nucleus in Saccharomyces cerevisiae. Furthermore, we were unable to detect chloroplast DNA-derived sequences among nuclear genome data for C. reinhardtii, which also contrasts with the situation in higher plants. Taken together, these findings suggest that there is presently little, if any, movement of DNA from chloroplast to nucleus in C. reinhardtii, which may reflect the ultrastructure of the C. reinhardtii cell.}, author = {Lister, Diane L. and Bateman, Joseph M. and Purton, Saul and Howe, Christopher J. }, citeulike-article-id = {1203260}, comment = {Bot\^{a}nica Marinha Aula: Endossimbioses}, doi = {http://dx.doi.org/10.1016/S0378-1119(03)00754-6}, journal = {Gene}, keywords = {endosymbiosis, evolution, plastids}, month = {October}, pages = {33--38}, posted-at = {2007-04-02 19:19:30}, priority = {0}, title = {DNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco}, url = {http://dx.doi.org/10.1016/S0378-1119(03)00754-6}, volume = {316}, year = {2003} }

TY - JOUR ID - citeulike:1203260 L3 - citeulike-article-id:1203260 N2 - By transforming chloroplasts with an antibiotic-resistance gene under the control of a nuclear-specific promoter, we employed a selection scheme to detect the transfer of DNA from the chloroplast to the nucleus in the green alga Chlamydomonas reinhardtii. Among several billion homoplasmic cells tested, we were unable to detect any stable nuclear integration of chloroplast DNA under normal growth conditions or under stress conditions. This contrasts with results reported for the transfer of DNA from chloroplast to nucleus in higher plants and from mitochondrion to nucleus in Saccharomyces cerevisiae. Furthermore, we were unable to detect chloroplast DNA-derived sequences among nuclear genome data for C. reinhardtii, which also contrasts with the situation in higher plants. Taken together, these findings suggest that there is presently little, if any, movement of DNA from chloroplast to nucleus in C. reinhardtii, which may reflect the ultrastructure of the C. reinhardtii cell. JF - Gene EP - 38 TI - DNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco VL - 316 SP - 33 KW - endosymbiosis KW - evolution KW - plastids N1 - Botânica Marinha Aula: Endossimbioses AU - Lister, Diane AU - Bateman, Joseph AU - Purton, Saul AU - Howe, Christopher PY - 2003/10/16/ UR - http://dx.doi.org/10.1016/S0378-1119(03)00754-6 ER -