Regulatory changes underlying expression differences within and between Drosophila species.

@article{citeulike:2420353, abstract = {Differences in gene expression are an important source of phenotypic variation, and can be caused by changes in cis and/or trans regulation. cis-regulatory variants alter allele-specific expression, whereas trans-regulatory variants influence expression of both alleles in a diploid cell. Because of this difference, we hypothesize that natural selection may favor one type of change over the other. Here, we investigate contributions of cis- and trans-regulatory changes to variable intra- and interspecific gene expression using four strains of Drosophila melanogaster, three strains of D. simulans and a total of 78 genes. We show that cis-regulatory changes account for a greater proportion of the expression differences observed between rather than within species. These data are inconsistent with a neutral model assuming equal probabilities of fixation for cis- and trans-regulatory polymorphisms, suggesting that natural selection influences the molecular mechanisms underlying divergent gene expression. Specifically, cis-regulatory changes seem to accumulate preferentially over time.}, address = {[1] Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA. [2] Department of Ecology and Evolutionary Biology, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA.}, author = {Wittkopp, Patricia J J. and Haerum, Belinda K K. and Clark, Andrew G G. }, citeulike-article-id = {2420353}, doi = {10.1038/ng.77}, issn = {1546-1718}, journal = {Nat Genet}, keywords = {allelic, drosophila, expression}, month = {February}, posted-at = {2008-02-28 19:14:51}, priority = {0}, title = {Regulatory changes underlying expression differences within and between Drosophila species.}, url = {http://dx.doi.org/10.1038/ng.77}, year = {2008} }

TY - JOUR ID - citeulike:2420353 L3 - citeulike-article-id:2420353 TI - Regulatory changes underlying expression differences within and between Drosophila species. JF - Nat Genet AD - [1] Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA. [2] Department of Ecology and Evolutionary Biology, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA. SN - 1546-1718 N2 - Differences in gene expression are an important source of phenotypic variation, and can be caused by changes in cis and/or trans regulation. cis-regulatory variants alter allele-specific expression, whereas trans-regulatory variants influence expression of both alleles in a diploid cell. Because of this difference, we hypothesize that natural selection may favor one type of change over the other. Here, we investigate contributions of cis- and trans-regulatory changes to variable intra- and interspecific gene expression using four strains of Drosophila melanogaster, three strains of D. simulans and a total of 78 genes. We show that cis-regulatory changes account for a greater proportion of the expression differences observed between rather than within species. These data are inconsistent with a neutral model assuming equal probabilities of fixation for cis- and trans-regulatory polymorphisms, suggesting that natural selection influences the molecular mechanisms underlying divergent gene expression. Specifically, cis-regulatory changes seem to accumulate preferentially over time. KW - allelic KW - drosophila KW - expression AU - Wittkopp, Patricia J AU - Haerum, Belinda K AU - Clark, Andrew G PY - 2008/02/17/ UR - http://dx.doi.org/10.1038/ng.77 ER -