Global identification of human transcribed sequences with genome tiling arrays.

@article{citeulike:396500, abstract = {Elucidating the transcribed regions of the genome constitutes a fundamental aspect of human biology, yet this remains an outstanding problem. To comprehensively identify coding sequences, we constructed a series of high-density oligonucleotide tiling arrays representing sense and antisense strands of the entire nonrepetitive sequence of the human genome. Transcribed sequences were located across the genome via hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained from human liver tissue. In addition to identifying many known and predicted genes, we found 10,595 transcribed sequences not detected by other methods. A large fraction of these are located in intergenic regions distal from previously annotated genes and exhibit significant homology to other mammalian proteins.}, address = {Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.}, author = {Bertone, P. and Stolc, V. and Royce, T. E. and Rozowsky, J. S. and Urban, A. E. and Zhu, X. and Rinn, J. L. and Tongprasit, W. and Samanta, M. and Weissman, S. and Gerstein, M. and Snyder, M. }, citeulike-article-id = {396500}, doi = {http://dx.doi.org/10.1126/science.1103388}, issn = {1095-9203}, journal = {Science}, keywords = {array, tiling}, month = {December}, number = {5705}, pages = {2242--2246}, posted-at = {2007-02-07 20:25:18}, priority = {2}, title = {Global identification of human transcribed sequences with genome tiling arrays.}, url = {http://dx.doi.org/10.1126/science.1103388}, volume = {306}, year = {2004} }

TY - JOUR ID - citeulike:396500 L3 - citeulike-article-id:396500 TI - Global identification of human transcribed sequences with genome tiling arrays. JF - Science VL - 306 IS - 5705 SP - 2242 EP - 2246 AD - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA. SN - 1095-9203 N2 - Elucidating the transcribed regions of the genome constitutes a fundamental aspect of human biology, yet this remains an outstanding problem. To comprehensively identify coding sequences, we constructed a series of high-density oligonucleotide tiling arrays representing sense and antisense strands of the entire nonrepetitive sequence of the human genome. Transcribed sequences were located across the genome via hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained from human liver tissue. In addition to identifying many known and predicted genes, we found 10,595 transcribed sequences not detected by other methods. A large fraction of these are located in intergenic regions distal from previously annotated genes and exhibit significant homology to other mammalian proteins. KW - array KW - tiling AU - Bertone, P AU - Stolc, V AU - Royce, TE AU - Rozowsky, JS AU - Urban, AE AU - Zhu, X AU - Rinn, JL AU - Tongprasit, W AU - Samanta, M AU - Weissman, S AU - Gerstein, M AU - Snyder, M PY - 2004/12/24/ UR - http://dx.doi.org/10.1126/science.1103388 ER -