GW body disassembly triggered by siRNAs independently of their silencing activity.

@article{citeulike:2649266, abstract = {GW bodies (or P-bodies) are cytoplasmic granules containing proteins involved in both mRNA degradation and storage, including the RNA interference machinery. Their mechanism of assembly and function are still poorly known although their number depends upon the flux of mRNA to be stored or degraded. We show here that silencing of the translational regulator CPEB1 leads to their disappearance, as reported for other GW body components. Surprisingly, the same results were obtained with several siRNAs targeting genes encoding proteins unrelated to mRNA metabolism. The disappearance of GW bodies did not correlate with the silencing activity of the siRNA and did not inhibit further silencing by siRNA. Importantly, in most cases, GW bodies were rapidly reinduced by arsenite, indicating that their assembly was not prevented by the inhibition of the targeted or off-target genes. We therefore propose that some siRNA sequences affect mRNA metabolism so as to diminish the amount of mRNA directed to the GW bodies. As an exception, GW bodies were not reinduced following Rck/p54 depletion by interference, indicating that this component is truly required for the GW body assembly. Noteworthy, Rck/p54 was dispensable for the assembly of stress granules, in spite of their close relationship with the GW bodies.}, address = {CNRS FRE2937, Institut Andr\'{e} Lwoff, 7 rue Guy Moquet, 94801 Villejuif cedex, France.}, author = {Serman, A. and Le Roy, F. and Aigueperse, C. and Kress, M. and Dautry, F. and Weil, D. }, citeulike-article-id = {2649266}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {stress\_granules, techniques}, number = {14}, pages = {4715--4727}, posted-at = {2008-04-10 14:29:45}, priority = {2}, title = {GW body disassembly triggered by siRNAs independently of their silencing activity.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17604308}, volume = {35}, year = {2007} }

TY - JOUR ID - citeulike:2649266 L3 - citeulike-article-id:2649266 TI - GW body disassembly triggered by siRNAs independently of their silencing activity. JF - Nucleic acids research VL - 35 IS - 14 SP - 4715 EP - 4727 AD - CNRS FRE2937, Institut André Lwoff, 7 rue Guy Moquet, 94801 Villejuif cedex, France. SN - 1362-4962 N2 - GW bodies (or P-bodies) are cytoplasmic granules containing proteins involved in both mRNA degradation and storage, including the RNA interference machinery. Their mechanism of assembly and function are still poorly known although their number depends upon the flux of mRNA to be stored or degraded. We show here that silencing of the translational regulator CPEB1 leads to their disappearance, as reported for other GW body components. Surprisingly, the same results were obtained with several siRNAs targeting genes encoding proteins unrelated to mRNA metabolism. The disappearance of GW bodies did not correlate with the silencing activity of the siRNA and did not inhibit further silencing by siRNA. Importantly, in most cases, GW bodies were rapidly reinduced by arsenite, indicating that their assembly was not prevented by the inhibition of the targeted or off-target genes. We therefore propose that some siRNA sequences affect mRNA metabolism so as to diminish the amount of mRNA directed to the GW bodies. As an exception, GW bodies were not reinduced following Rck/p54 depletion by interference, indicating that this component is truly required for the GW body assembly. Noteworthy, Rck/p54 was dispensable for the assembly of stress granules, in spite of their close relationship with the GW bodies. KW - stress_granules KW - techniques AU - Serman, A AU - Le Roy, F AU - Aigueperse, C AU - Kress, M AU - Dautry, F AU - Weil, D PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/17604308 ER -