Hybrid alphavirus-rhabdovirus propagating replicon particles are versatile and potent vaccine vectors

@article{citeulike:2648231, abstract = {Self-propagating, infectious, virus-like particles are generated in animal cell lines transfected with a Semliki Forest virus RNA replicon encoding a single viral structural protein, the vesicular stomatitis virus (VSV) glycoprotein. We show here that these infectious particles, which we call propagating replicons, are potent inducers of neutralizing antibody in animals yet are nonpathogenic. Mice vaccinated with a single dose of the particles generated high titers of VSV-neutralizing antibody and were protected from a subsequent lethal challenge with VSV. Induction of antibody required RNA replication. We also report that additional genes (including an HIV-1 envelope protein gene) expressed from the propagating replicons induced strong cellular immune responses to the corresponding proteins after a single inoculation. Our studies reveal the potential of these particles as simple and safe vaccine vectors inducing strong humoral and cellular immune responses. 10.1073/pnas.0800280105}, author = {Rose, Nina F. and Publicover, Jean and Chattopadhyay, Anasuya and Rose, John K. }, citeulike-article-id = {2648231}, doi = {http://dx.doi.org/10.1073/pnas.0800280105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {treatment}, month = {April}, pages = {0800280105+}, posted-at = {2008-04-10 08:17:20}, priority = {2}, title = {Hybrid alphavirus-rhabdovirus propagating replicon particles are versatile and potent vaccine vectors}, url = {http://dx.doi.org/10.1073/pnas.0800280105}, year = {2008} }

TY - JOUR ID - citeulike:2648231 L3 - citeulike-article-id:2648231 N2 - Self-propagating, infectious, virus-like particles are generated in animal cell lines transfected with a Semliki Forest virus RNA replicon encoding a single viral structural protein, the vesicular stomatitis virus (VSV) glycoprotein. We show here that these infectious particles, which we call propagating replicons, are potent inducers of neutralizing antibody in animals yet are nonpathogenic. Mice vaccinated with a single dose of the particles generated high titers of VSV-neutralizing antibody and were protected from a subsequent lethal challenge with VSV. Induction of antibody required RNA replication. We also report that additional genes (including an HIV-1 envelope protein gene) expressed from the propagating replicons induced strong cellular immune responses to the corresponding proteins after a single inoculation. Our studies reveal the potential of these particles as simple and safe vaccine vectors inducing strong humoral and cellular immune responses. 10.1073/pnas.0800280105 JF - Proceedings of the National Academy of Sciences TI - Hybrid alphavirus-rhabdovirus propagating replicon particles are versatile and potent vaccine vectors SP - 0800280105 KW - treatment AU - Rose, Nina AU - Publicover, Jean AU - Chattopadhyay, Anasuya AU - Rose, John PY - 2008/04/07/ UR - http://dx.doi.org/10.1073/pnas.0800280105 ER -