Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.

by: AJ Nataraj, I Olivos-Glander, N Kusukawa, WE Highsmith

Electrophoresis, Vol. 20, No. 6. (June 1999), pp. 1177-1185.

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Abstract

Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and their relatively high sensitivity for the detection of mutations. Here we review the theory and practice of SSCP and HA, including the factors contributing to the sensitivity of mutation detection. For SSCP analysis, these factors include: choice of gel matrix, electrophoretic conditions, presence of neutral additives, fragment size, and G+C content For HA, the principle factors influencing sensitivity are the gel matrix and the identity of the base mismatch.

@article{citeulike:570671, abstract = {Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and their relatively high sensitivity for the detection of mutations. Here we review the theory and practice of SSCP and HA, including the factors contributing to the sensitivity of mutation detection. For SSCP analysis, these factors include: choice of gel matrix, electrophoretic conditions, presence of neutral additives, fragment size, and G+C content For HA, the principle factors influencing sensitivity are the gel matrix and the identity of the base mismatch.}, address = {FMC BioProducts, Rockland, ME, USA.}, author = {Nataraj, A. J. and Olivos-Glander, I. and Kusukawa, N. and Highsmith, W. E. }, citeulike-article-id = {570671}, doi = {10.1002/(SICI)1522-2683(19990101)20:6<1177::AID-ELPS1177>3.0.CO;2-2}, issn = {0173-0835}, journal = {Electrophoresis}, keywords = {rflp, snp}, month = {June}, number = {6}, pages = {1177--1185}, posted-at = {2008-07-23 17:45:21}, priority = {2}, title = {Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.}, url = {http://dx.doi.org/10.1002/(SICI)1522-2683(19990101)20:6\%3C1177::AID-ELPS1177\%3E3.0.CO;2-2}, volume = {20}, year = {1999} }

RIS record

TY - JOUR ID - citeulike:570671 L3 - citeulike-article-id:570671 TI - Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection. JF - Electrophoresis VL - 20 IS - 6 SP - 1177 EP - 1185 AD - FMC BioProducts, Rockland, ME, USA. SN - 0173-0835 N2 - Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and their relatively high sensitivity for the detection of mutations. Here we review the theory and practice of SSCP and HA, including the factors contributing to the sensitivity of mutation detection. For SSCP analysis, these factors include: choice of gel matrix, electrophoretic conditions, presence of neutral additives, fragment size, and G+C content For HA, the principle factors influencing sensitivity are the gel matrix and the identity of the base mismatch. KW - rflp KW - snp AU - Nataraj, AJ AU - Olivos-Glander, I AU - Kusukawa, N AU - Highsmith, WE PY - 1999/06// UR - http://dx.doi.org/10.1002/(SICI)1522-2683(19990101)20:6%3C1177::AID-ELPS1177%3E3.0.CO;2-2 ER -

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