Normalization of full-length enriched cDNA.

by: EA Bogdanova, DA Shagin, SA Lukyanov

Molecular bioSystems, Vol. 4, No. 3. (March 2008), pp. 205-212.

View FullText article

DOI, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

jyuh

Find related articles with these CiteULike tags

plasmid

Abstract

Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normalization methods have been developed since 1990. A number of these methods have been optimized for the normalization of full-length enriched cDNA, and used in various applications, including transcriptome analysis and functional screening of cDNA libraries. One such procedure (named DSN-normalization) is based on the unique properties of duplex-specific nuclease (DSN) from kamchatka crab and allows the generation of normalized cDNA libraries with a high gene discovery rate.

@article{citeulike:2925069, abstract = {Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normalization methods have been developed since 1990. A number of these methods have been optimized for the normalization of full-length enriched cDNA, and used in various applications, including transcriptome analysis and functional screening of cDNA libraries. One such procedure (named DSN-normalization) is based on the unique properties of duplex-specific nuclease (DSN) from kamchatka crab and allows the generation of normalized cDNA libraries with a high gene discovery rate.}, address = {Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry, 16/10 Miklukho-Maklaya, Moscow, Russia.}, author = {Bogdanova, E. A. and Shagin, D. A. and Lukyanov, S. A. }, citeulike-article-id = {2925069}, doi = {10.1039/b715110c}, issn = {1742-206X}, journal = {Molecular bioSystems}, keywords = {plasmid}, month = {March}, number = {3}, pages = {205--212}, posted-at = {2008-06-25 06:39:24}, priority = {2}, title = {Normalization of full-length enriched cDNA.}, url = {http://dx.doi.org/10.1039/b715110c}, volume = {4}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2925069 L3 - citeulike-article-id:2925069 TI - Normalization of full-length enriched cDNA. JF - Molecular bioSystems VL - 4 IS - 3 SP - 205 EP - 212 AD - Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry, 16/10 Miklukho-Maklaya, Moscow, Russia. SN - 1742-206X N2 - Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normalization methods have been developed since 1990. A number of these methods have been optimized for the normalization of full-length enriched cDNA, and used in various applications, including transcriptome analysis and functional screening of cDNA libraries. One such procedure (named DSN-normalization) is based on the unique properties of duplex-specific nuclease (DSN) from kamchatka crab and allows the generation of normalized cDNA libraries with a high gene discovery rate. KW - plasmid AU - Bogdanova, EA AU - Shagin, DA AU - Lukyanov, SA PY - 2008/03// UR - http://dx.doi.org/10.1039/b715110c ER -

RIS BibTeX