Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides

@article{citeulike:2904399, abstract = {The potential use of antisense and siRNA oligonucleotides as therapeutic agents has elicited a great deal of interest. However, a major issue for oligonucleotide-based therapeutics involves effective intracellular delivery of the active molecules. In this Survey and Summary, we review recent reports on delivery strategies, including conjugates of oligonucleotides with various ligands, as well as use of nanocarrier approaches. These are discussed in the context of intracellular trafficking pathways and issues regarding in vivo biodistribution of molecules and nanoparticles. Molecular-sized chemical conjugates and supramolecular nanocarriers each display advantages and disadvantages in terms of effective and nontoxic delivery. Thus, choice of an optimal delivery modality will likely depend on the therapeutic context. 10.1093/nar/gkn342}, author = {Juliano, Rudy and Alam, Md and Dixit, Vidula and Kang, Hyumin }, citeulike-article-id = {2904399}, doi = {10.1093/nar/gkn342}, journal = {Nucl. Acids Res.}, keywords = {antisense, delivery, rnai}, month = {June}, pages = {gkn342+}, posted-at = {2008-06-18 08:34:53}, priority = {2}, title = {Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides}, url = {http://dx.doi.org/10.1093/nar/gkn342}, year = {2008} }

TY - JOUR ID - citeulike:2904399 L3 - citeulike-article-id:2904399 TI - Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides JF - Nucl. Acids Res. SP - gkn342 N2 - The potential use of antisense and siRNA oligonucleotides as therapeutic agents has elicited a great deal of interest. However, a major issue for oligonucleotide-based therapeutics involves effective intracellular delivery of the active molecules. In this Survey and Summary, we review recent reports on delivery strategies, including conjugates of oligonucleotides with various ligands, as well as use of nanocarrier approaches. These are discussed in the context of intracellular trafficking pathways and issues regarding in vivo biodistribution of molecules and nanoparticles. Molecular-sized chemical conjugates and supramolecular nanocarriers each display advantages and disadvantages in terms of effective and nontoxic delivery. Thus, choice of an optimal delivery modality will likely depend on the therapeutic context. 10.1093/nar/gkn342 KW - antisense KW - delivery KW - rnai AU - Juliano, Rudy AU - Alam, Md AU - Dixit, Vidula AU - Kang, Hyumin PY - 2008/06/16/ UR - http://dx.doi.org/10.1093/nar/gkn342 ER -