MicroRNA expression during chick embryo development

@article{citeulike:883707, abstract = {MicroRNAs (miRNAs) are small, abundant, noncoding RNAs that modulate protein abundance by interfering with target mRNA translation or stability. miRNAs are detected in organisms from all domains and may regulate 30\% of transcripts in vertebrates. Understanding miRNA function requires a detailed determination of expression, yet this has not been reported in an amniote species. High-throughput whole mount in situ hybridization was performed on chicken embryos to map expression of 135 miRNA genes including five miRNAs that had not been previously reported in chicken. Eighty-four miRNAs were detected before day 5 of embryogenesis, and 75 miRNAs showed differential expression. Whereas few miRNAs were expressed during formation of the primary germ layers, the number of miRNAs detected increased rapidly during organogenesis. Patterns highlighted cell-type, organ or structure-specific expression, localization within germ layers and their derivatives, and expression in multiple cell and tissue types and within sub-regions of structures and tissues. A novel group of miRNAs was highly expressed in most tissues but much reduced in one or a few organs, including the heart. This study presents the first comprehensive overview of miRNA expression in an amniote organism and provides an important foundation for investigations of miRNA gene regulation and function. Developmental Dynamics, 2006. {\copyright} 2006 Wiley-Liss, Inc.}, address = {Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona; Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona}, author = {Darnell, Diana K. and Kaur, Simran and Stanislaw, Stacey and Konieczka, Jay K. and Yatskievych, Tatiana A. and Antin, Parker B. }, citeulike-article-id = {883707}, doi = {10.1002/dvdy.20956}, journal = {Developmental Dynamics}, keywords = {microrna, microrna\_expression}, number = {9999}, pages = {NA+}, posted-at = {2008-06-30 13:11:11}, priority = {2}, title = {MicroRNA expression during chick embryo development}, url = {http://dx.doi.org/10.1002/dvdy.20956}, volume = {9999}, year = {2006} }

TY - JOUR ID - citeulike:883707 L3 - citeulike-article-id:883707 TI - MicroRNA expression during chick embryo development JF - Developmental Dynamics VL - 9999 IS - 9999 SP - NA AD - Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona; Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona N2 - MicroRNAs (miRNAs) are small, abundant, noncoding RNAs that modulate protein abundance by interfering with target mRNA translation or stability. miRNAs are detected in organisms from all domains and may regulate 30% of transcripts in vertebrates. Understanding miRNA function requires a detailed determination of expression, yet this has not been reported in an amniote species. High-throughput whole mount in situ hybridization was performed on chicken embryos to map expression of 135 miRNA genes including five miRNAs that had not been previously reported in chicken. Eighty-four miRNAs were detected before day 5 of embryogenesis, and 75 miRNAs showed differential expression. Whereas few miRNAs were expressed during formation of the primary germ layers, the number of miRNAs detected increased rapidly during organogenesis. Patterns highlighted cell-type, organ or structure-specific expression, localization within germ layers and their derivatives, and expression in multiple cell and tissue types and within sub-regions of structures and tissues. A novel group of miRNAs was highly expressed in most tissues but much reduced in one or a few organs, including the heart. This study presents the first comprehensive overview of miRNA expression in an amniote organism and provides an important foundation for investigations of miRNA gene regulation and function. Developmental Dynamics, 2006. © 2006 Wiley-Liss, Inc. KW - microrna KW - microrna_expression AU - Darnell, Diana AU - Kaur, Simran AU - Stanislaw, Stacey AU - Konieczka, Jay AU - Yatskievych, Tatiana AU - Antin, Parker PY - 2006/// UR - http://dx.doi.org/10.1002/dvdy.20956 ER -