NblR is a novel one-component response regulator in the cyanobacterium Synechococcus elongatus PCC 7942.

@article{citeulike:2783155, abstract = {A response regulator, NblR, of the cyanobacterium Synechococcus elongatus PCC 7942 is known to induce expression of the nblA gene, a key factor in phycobilisome degradation (bleaching) under nutrient-deprivation conditions. In this study, we observed phosphorylation-independent regulation of NblR activity. We constructed a mutant strain expressing NblR (D57A), in which a putative phospho-accepting Asp-57 was replaced with Ala residue. Under nitrogen deprivation, this strain exhibited the typical bleaching phenotype observed in wild-type cells. Moreover, in the mutant, the nblA transcript accumulated at a level similar to that of the wild type. Our results indicate that activation of NblR is independent of phosphorylation, if any, by a cognate histidine kinase. Screening of proteins interacting with NblR by yeast two-hybrid analysis revealed two candidates, MreC and NarB, suggesting a novel mechanism that activates NblR, or other functions of the response regulator.}, address = {Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.}, author = {Kato, H. and Chibazakura, T. and Yoshikawa, H. }, citeulike-article-id = {2783155}, issn = {1347-6947}, journal = {Bioscience, biotechnology, and biochemistry}, keywords = {nblr, pcc7942}, month = {April}, number = {4}, pages = {1072--1079}, posted-at = {2008-05-10 19:58:36}, priority = {2}, title = {NblR is a novel one-component response regulator in the cyanobacterium Synechococcus elongatus PCC 7942.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18391440}, volume = {72}, year = {2008} }

TY - JOUR ID - citeulike:2783155 L3 - citeulike-article-id:2783155 TI - NblR is a novel one-component response regulator in the cyanobacterium Synechococcus elongatus PCC 7942. JF - Bioscience, biotechnology, and biochemistry VL - 72 IS - 4 SP - 1072 EP - 1079 AD - Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. SN - 1347-6947 N2 - A response regulator, NblR, of the cyanobacterium Synechococcus elongatus PCC 7942 is known to induce expression of the nblA gene, a key factor in phycobilisome degradation (bleaching) under nutrient-deprivation conditions. In this study, we observed phosphorylation-independent regulation of NblR activity. We constructed a mutant strain expressing NblR (D57A), in which a putative phospho-accepting Asp-57 was replaced with Ala residue. Under nitrogen deprivation, this strain exhibited the typical bleaching phenotype observed in wild-type cells. Moreover, in the mutant, the nblA transcript accumulated at a level similar to that of the wild type. Our results indicate that activation of NblR is independent of phosphorylation, if any, by a cognate histidine kinase. Screening of proteins interacting with NblR by yeast two-hybrid analysis revealed two candidates, MreC and NarB, suggesting a novel mechanism that activates NblR, or other functions of the response regulator. KW - nblr KW - pcc7942 AU - Kato, H AU - Chibazakura, T AU - Yoshikawa, H PY - 2008/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/18391440 ER -