BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.

@article{citeulike:2944693, abstract = {We report the identities of the members of a group of proteins that associate with BRCA1 to form a large complex that we have named BASC (BRCA1-associated genome surveillance complex). This complex includes tumor suppressors and DNA damage repair proteins MSH2, MSH6, MLH1, ATM, BLM, and the RAD50-MRE11-NBS1 protein complex. In addition, DNA replication factor C (RFC), a protein complex that facilitates the loading of PCNA onto DNA, is also part of BASC. We find that BRCA1, the BLM helicase, and the RAD50-MRE11-NBS1 complex colocalize to large nuclear foci that contain PCNA when cells are treated with agents that interfere with DNA synthesis. The association of BRCA1 with MSH2 and MSH6, which are required for transcription-coupled repair, provides a possible explanation for the role of BRCA1 in this pathway. Strikingly, all members of this complex have roles in recognition of abnormal DNA structures or damaged DNA, suggesting that BASC may serve as a sensor for DNA damage. Several of these proteins also have roles in DNA replication-associated repair. Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair.}, address = {Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.}, author = {Wang, Y. and Cortez, D. and Yazdi, P. and Neff, N. and Elledge, S. J. and Qin, J. }, citeulike-article-id = {2944693}, issn = {0890-9369}, journal = {Genes \& development}, keywords = {basc, methods, protein}, month = {April}, number = {8}, pages = {927--939}, posted-at = {2008-06-30 14:57:34}, priority = {2}, title = {BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10783165}, volume = {14}, year = {2000} }

TY - JOUR ID - citeulike:2944693 L3 - citeulike-article-id:2944693 TI - BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. JF - Genes & development VL - 14 IS - 8 SP - 927 EP - 939 AD - Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA. SN - 0890-9369 N2 - We report the identities of the members of a group of proteins that associate with BRCA1 to form a large complex that we have named BASC (BRCA1-associated genome surveillance complex). This complex includes tumor suppressors and DNA damage repair proteins MSH2, MSH6, MLH1, ATM, BLM, and the RAD50-MRE11-NBS1 protein complex. In addition, DNA replication factor C (RFC), a protein complex that facilitates the loading of PCNA onto DNA, is also part of BASC. We find that BRCA1, the BLM helicase, and the RAD50-MRE11-NBS1 complex colocalize to large nuclear foci that contain PCNA when cells are treated with agents that interfere with DNA synthesis. The association of BRCA1 with MSH2 and MSH6, which are required for transcription-coupled repair, provides a possible explanation for the role of BRCA1 in this pathway. Strikingly, all members of this complex have roles in recognition of abnormal DNA structures or damaged DNA, suggesting that BASC may serve as a sensor for DNA damage. Several of these proteins also have roles in DNA replication-associated repair. Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair. KW - basc KW - methods KW - protein AU - Wang, Y AU - Cortez, D AU - Yazdi, P AU - Neff, N AU - Elledge, SJ AU - Qin, J PY - 2000/04/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10783165 ER -