The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120

@article{citeulike:2775215, abstract = {The gene hetN encodes a putative oxidoreductase that is known to suppress heterocyst differentiation when present on a multicopy plasmid in Anabaena sp. PCC 7120. To mimic the hetN null phenotype and to examine where HetN acts in the regulatory cascade that controls heterocyst differentiation, we replaced the native chromosomal hetN promoter with the copper-inducible petE promoter. In the presence of copper, heterocyst formation was suppressed in undifferentiated filaments. When hetN expression was turned off by transferring cells to media lacking copper, the filaments initially displayed the wild-type pattern of single heterocysts but, 48 h after the induction of heterocyst formation, a pattern of multiple contiguous heterocysts predominated. Suppression of heterocyst formation by HetN appears to occur both upstream and downstream of the positive regulator HetR: overexpression of hetN in undifferentiated filaments prevents the wild-type pattern of hetR expression as well as the multiheterocyst phenotype normally observed when hetR is expressed from an inducible promoter. Green fluorescent protein fusions show that the expression of hetN in wild-type filaments normally occurs primarily in heterocysts. We propose that HetN is normally involved in the maintenance of heterocyst spacing after the initial heterocyst pattern has been established, but ectopic expression of hetN can also block the initial establishment of the pattern.}, author = {Callahan, Sean M. and Buikema, William J. }, citeulike-article-id = {2775215}, doi = {10.1046/j.1365-2958.2001.02437.x}, journal = {Molecular Microbiology}, keywords = {biochempaper, heterocyst, hetn}, number = {4}, pages = {941--950}, posted-at = {2008-05-09 11:42:13}, priority = {0}, title = {The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120}, url = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2001.02437.x}, volume = {40}, year = {2001} }

TY - JOUR ID - citeulike:2775215 L3 - citeulike-article-id:2775215 TI - The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120 JF - Molecular Microbiology VL - 40 IS - 4 SP - 941 EP - 950 N2 - The gene hetN encodes a putative oxidoreductase that is known to suppress heterocyst differentiation when present on a multicopy plasmid in Anabaena sp. PCC 7120. To mimic the hetN null phenotype and to examine where HetN acts in the regulatory cascade that controls heterocyst differentiation, we replaced the native chromosomal hetN promoter with the copper-inducible petE promoter. In the presence of copper, heterocyst formation was suppressed in undifferentiated filaments. When hetN expression was turned off by transferring cells to media lacking copper, the filaments initially displayed the wild-type pattern of single heterocysts but, 48 h after the induction of heterocyst formation, a pattern of multiple contiguous heterocysts predominated. Suppression of heterocyst formation by HetN appears to occur both upstream and downstream of the positive regulator HetR: overexpression of hetN in undifferentiated filaments prevents the wild-type pattern of hetR expression as well as the multiheterocyst phenotype normally observed when hetR is expressed from an inducible promoter. Green fluorescent protein fusions show that the expression of hetN in wild-type filaments normally occurs primarily in heterocysts. We propose that HetN is normally involved in the maintenance of heterocyst spacing after the initial heterocyst pattern has been established, but ectopic expression of hetN can also block the initial establishment of the pattern. KW - biochempaper KW - heterocyst KW - hetn AU - Callahan, Sean AU - Buikema, William PY - 2001/// UR - http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2001.02437.x ER -