Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120.

@article{citeulike:2725158, abstract = {Anabaena 7120 mutant 216 fails to differentiate heterocyst. We previously identified a 2.4-kb wild-type DNA fragment able to complement this mutant. We show here that the sequence of this fragment contains a single open reading frame (hetR), encoding a 299-amino-acid protein. Conjugation of deletion subclones of this fragment into strain 216 showed that the hetR-coding region is both necessary and sufficient for complementation of the Het- phenotype. The mutation in 216 is located at nucleotide 535 in the hetR gene, converting a serine at position 179 in the wild-type protein to an asparagine in the mutant. Interruption of the hetR gene in wild-type cells results in a mutant phenotype identical to that of 216. Both 216 and wild-type cells containing wild-type hetR on a plasmid display increased frequency of heterocysts, even on media containing fixed nitrogen. These results suggest that hetR encodes a product that is not only essential for but also controls heterocyst development. This putative regulatory protein lacks known structural motifs characteristic of transcription factors and probably acts at a level one or more steps removed from its target genes. 10.1101/gad.5.2.321}, author = {Buikema, W. J. and Haselkorn, R. }, citeulike-article-id = {2725158}, doi = {10.1101/gad.5.2.321}, journal = {Genes Dev.}, keywords = {biochempaper, heterocyst}, month = {February}, number = {2}, pages = {321--330}, posted-at = {2008-04-28 00:04:33}, priority = {2}, title = {Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120.}, url = {http://dx.doi.org/10.1101/gad.5.2.321}, volume = {5}, year = {1991} }

TY - JOUR ID - citeulike:2725158 L3 - citeulike-article-id:2725158 TI - Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. JF - Genes Dev. VL - 5 IS - 2 SP - 321 EP - 330 N2 - Anabaena 7120 mutant 216 fails to differentiate heterocyst. We previously identified a 2.4-kb wild-type DNA fragment able to complement this mutant. We show here that the sequence of this fragment contains a single open reading frame (hetR), encoding a 299-amino-acid protein. Conjugation of deletion subclones of this fragment into strain 216 showed that the hetR-coding region is both necessary and sufficient for complementation of the Het- phenotype. The mutation in 216 is located at nucleotide 535 in the hetR gene, converting a serine at position 179 in the wild-type protein to an asparagine in the mutant. Interruption of the hetR gene in wild-type cells results in a mutant phenotype identical to that of 216. Both 216 and wild-type cells containing wild-type hetR on a plasmid display increased frequency of heterocysts, even on media containing fixed nitrogen. These results suggest that hetR encodes a product that is not only essential for but also controls heterocyst development. This putative regulatory protein lacks known structural motifs characteristic of transcription factors and probably acts at a level one or more steps removed from its target genes. 10.1101/gad.5.2.321 KW - biochempaper KW - heterocyst AU - Buikema, WJ AU - Haselkorn, R PY - 1991/02/01/ UR - http://dx.doi.org/10.1101/gad.5.2.321 ER -