The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development

@article{citeulike:2679233, abstract = {The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the hetR and hetC genes. Expression of hetC, tested by RNA- DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide [-]571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of hetR expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for hetR induction.}, author = {Muro-Pastor, Alicia M. and Valladares, Ana and Flores, Enrique and Herrero, Antonia }, citeulike-article-id = {2679233}, journal = {J. Bacteriol.}, keywords = {biochempaper, heterocyst}, month = {November}, number = {21}, pages = {6664--6669}, posted-at = {2008-04-17 01:24:51}, priority = {2}, title = {The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development}, url = {http://jb.asm.org/cgi/content/abstract/181/21/6664}, volume = {181}, year = {1999} }

TY - JOUR ID - citeulike:2679233 L3 - citeulike-article-id:2679233 TI - The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development JF - J. Bacteriol. VL - 181 IS - 21 SP - 6664 EP - 6669 N2 - The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the hetR and hetC genes. Expression of hetC, tested by RNA- DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide [-]571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of hetR expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for hetR induction. KW - biochempaper KW - heterocyst AU - Muro-Pastor, Alicia AU - Valladares, Ana AU - Flores, Enrique AU - Herrero, Antonia PY - 1999/11/01/ UR - http://jb.asm.org/cgi/content/abstract/181/21/6664 ER -