Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy.

@article{citeulike:1652078, abstract = {Sequences of the variable intergenic spacer region 5S (rrfA) 23S (rrlB) rRNA were used to identify Borrelia genospecies present in Ixodes ricinus nymphs collected from the Lamar Lakes area of the Province of Trento, Italy (overall prevalence=6.3\%). Four genospecies were identified, one for the first time in this Province (B. valaisiana), and three which have been noted previously (B. afzelii, B. garinii, and B. burgdorferi s.s.). In order to compare the genetic variability of these genospecies in Trento with that at a European level, our 21 sequences (15 new haplotypes) and all appropriate European Borrelia sequences registered in GenBank (up to the end of 2004) were subjected to a phylogenetic analysis (for a total of 73 sequences and 43 haplotypes). Clusters of sequences representing the five main European genospecies (afzelii, garinii, burgdorferi s.s., valaisiana, lusitaniae) are well-supported. At least two other groups of haplotypes (genospecies) are suggested by our analysis; moreover, divergent evolution may be occurring in several genospecies. The maximum uncorrected pairwise differences between sequences within genospecies ranges from 1.5\% (B. burgdorferi s.s.), to 2.3\% (B. garinii and B. valaisiana) to 4.7\% (B. afzelii), and are not correlated with geographical distribution. Within the Province of Trento, these values for the same genospecies are 1.5\%, 2.3\%, 0.9\%, 1.9\%, respectively. These high mutation rates within genospecies suggest that the sequencing of haplotypes should continue if we are to fully understand and monitor the evolution and epidemiology of Borrelia.}, address = {Centre for Alpine Ecology, I-38040 Viote del Monte Bondone, Trento, Italy. pecchioli@cealp.it}, author = {Pecchioli, E. and Hauffe, H. C. and Tagliapietra, V. and Bandi, C. and Genchi, C. and Rizzoli, A. }, citeulike-article-id = {1652078}, doi = {http://dx.doi.org/10.1016/j.ijmm.2006.07.003}, issn = {1438-4221}, journal = {Int J Med Microbiol}, keywords = {borrelia, genetic\_variability, ixodes}, month = {February}, number = {1}, pages = {53--59}, posted-at = {2007-09-13 15:39:09}, priority = {2}, title = {Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy.}, url = {http://dx.doi.org/10.1016/j.ijmm.2006.07.003}, volume = {297}, year = {2007} }

TY - JOUR ID - citeulike:1652078 L3 - citeulike-article-id:1652078 N2 - Sequences of the variable intergenic spacer region 5S (rrfA) 23S (rrlB) rRNA were used to identify Borrelia genospecies present in Ixodes ricinus nymphs collected from the Lamar Lakes area of the Province of Trento, Italy (overall prevalence=6.3%). Four genospecies were identified, one for the first time in this Province (B. valaisiana), and three which have been noted previously (B. afzelii, B. garinii, and B. burgdorferi s.s.). In order to compare the genetic variability of these genospecies in Trento with that at a European level, our 21 sequences (15 new haplotypes) and all appropriate European Borrelia sequences registered in GenBank (up to the end of 2004) were subjected to a phylogenetic analysis (for a total of 73 sequences and 43 haplotypes). Clusters of sequences representing the five main European genospecies (afzelii, garinii, burgdorferi s.s., valaisiana, lusitaniae) are well-supported. At least two other groups of haplotypes (genospecies) are suggested by our analysis; moreover, divergent evolution may be occurring in several genospecies. The maximum uncorrected pairwise differences between sequences within genospecies ranges from 1.5% (B. burgdorferi s.s.), to 2.3% (B. garinii and B. valaisiana) to 4.7% (B. afzelii), and are not correlated with geographical distribution. Within the Province of Trento, these values for the same genospecies are 1.5%, 2.3%, 0.9%, 1.9%, respectively. These high mutation rates within genospecies suggest that the sequencing of haplotypes should continue if we are to fully understand and monitor the evolution and epidemiology of Borrelia. IS - 1 JF - Int J Med Microbiol SN - 1438-4221 AD - Centre for Alpine Ecology, I-38040 Viote del Monte Bondone, Trento, Italy. pecchioli@cealp.it EP - 59 TI - Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy. VL - 297 SP - 53 KW - borrelia KW - genetic_variability KW - ixodes AU - Pecchioli, E AU - Hauffe, HC AU - Tagliapietra, V AU - Bandi, C AU - Genchi, C AU - Rizzoli, A PY - 2007/02// UR - http://dx.doi.org/10.1016/j.ijmm.2006.07.003 ER -