Genomic mapping by fingerprinting random clones: a mathematical analysis.

@article{citeulike:163579, abstract = {Results from physical mapping projects have recently been reported for the genomes of Escherichia coli, Saccharomyces cerevisiae, and Caenorhabditis elegans, and similar projects are currently being planned for other organisms. In such projects, the physical map is assembled by first "fingerprinting" a large number of clones chosen at random from a recombinant library and then inferring overlaps between clones with sufficiently similar fingerprints. Although the basic approach is the same, there are many possible choices for the fingerprint used to characterize the clones and the rules for declaring overlap. In this paper, we derive simple formulas showing how the progress of a physical mapping project is affected by the nature of the fingerprinting scheme. Using these formulas, we discuss the analytic considerations involved in selecting an appropriate fingerprinting scheme for a particular project.}, address = {Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142.}, author = {Lander, E. S. and Waterman, M. S. }, citeulike-article-id = {163579}, issn = {0888-7543}, journal = {Genomics}, keywords = {assembly, coverage, wgs}, month = {April}, number = {3}, pages = {231--239}, posted-at = {2008-05-19 13:22:57}, priority = {2}, title = {Genomic mapping by fingerprinting random clones: a mathematical analysis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3294162}, volume = {2}, year = {1988} }

TY - JOUR ID - citeulike:163579 L3 - citeulike-article-id:163579 TI - Genomic mapping by fingerprinting random clones: a mathematical analysis. JF - Genomics VL - 2 IS - 3 SP - 231 EP - 239 AD - Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142. SN - 0888-7543 N2 - Results from physical mapping projects have recently been reported for the genomes of Escherichia coli, Saccharomyces cerevisiae, and Caenorhabditis elegans, and similar projects are currently being planned for other organisms. In such projects, the physical map is assembled by first "fingerprinting" a large number of clones chosen at random from a recombinant library and then inferring overlaps between clones with sufficiently similar fingerprints. Although the basic approach is the same, there are many possible choices for the fingerprint used to characterize the clones and the rules for declaring overlap. In this paper, we derive simple formulas showing how the progress of a physical mapping project is affected by the nature of the fingerprinting scheme. Using these formulas, we discuss the analytic considerations involved in selecting an appropriate fingerprinting scheme for a particular project. KW - assembly KW - coverage KW - wgs AU - Lander, ES AU - Waterman, MS PY - 1988/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/3294162 ER -